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Abstract Detail


Chau, John [1], Olmstead, Richard [1].

Comparison of taxon-specific versus general locus sets for targeted sequence capture in plant phylogenomics.

Targeted sequence capture can be used to efficiently gather sequence data for large numbers of loci, such as single-copy nuclear loci. Most published studies in plants have used taxon-specific locus sets developed individually for a clade using multiple genomic and transcriptomic resources. General locus sets can also be developed from loci that have been identified as single-copy and having orthologs in large clades of plants. We identify and compare a taxon-specific locus set and three general locus sets (COSII, APVO SSC, PPR) for targeted sequence capture in Buddleja (Scrophulariaceae) and outgroups. We evaluate their performance in terms of assembly success, sequence variability, and resolution and support of inferred phylogenetic trees.

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1 - University of Washington, Department of Biology, Box 351800, Seattle, WA, 98195, USA

hybrid enrichment
single-copy nuclear genes
PPR genes

Presentation Type: Oral Paper
Session: 1, Phylogenomics I
Location: 114/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 8:00 AM
Number: 1001
Abstract ID:111
Candidate for Awards:None

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