Abstract Detail



Comparative Genomics/Transcriptomics

Lawrence , Amelia H [1], Kinser, Taliesin [2], Puzey, Joshua [3].

Methylation repatterning in the endosperm and embryo of interploid Mimulus hybrids.

Plant hybridization offers a unique window to explore genomic interactions between divergent genomes.  One potential manifestation of interactions between genomes in hybrid individuals is methylation repatterning. To understand the impact the interploid hybridization has on methylation repatterning we utilized Mimulus as a model system. Mimulus is useful because it has species of varying ploidies and its genome is fully sequenced. Because the endosperm and embryo are the first areas where these genomes interact, we examine methylation repatterning in the context of these tissues. Using Mimulus we examined endosperm and embryo methylation in reciprocal crosses between M. guttatus (2n) and M.luteus (4n) (2n x 4n and 4n x 2n) as well as parental crosses (2n x 2n and 4n x 4n). Overall, we find that all types of methylation (CG, CHH, CHG) are significantly repatterned in the hybrid endosperm and embryo. These results have broad implications for understanding genomic imprinting, seed development, and hybrid incompatibility.


Related Links:
Puzey Lab Homepage


1 - College of William & Mary, Biology, 540 Landrum Dr., 110 Sadler Center, Williamsburg, Virginia, 23185, United States
2 - College of William & Mary, 540 Landrum Dr., Williamsburg, Virginia, 23185, United States
3 - College Of William And Mary, Biology Dept., 540 Landrum Dr., Williamsburg, VA, 23185, United States

Keywords:
Methylation
Endosperm
Hybridization
Polyploid
Interploid
Mimulus.

Presentation Type: Poster
Number: PGT008
Abstract ID:282
Candidate for Awards:Genetics Section Poster Award


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