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Abstract Detail


Ogbimi, Ejeoghene [1], Sakpere, Ayobola [2].

Shoot Regeneration and Callus Induction Potential of Node and Cotyledonary node Explants from In vitro Grown Seedlings of Afzelia africana – An Important yet Threatened Legume.

Afzelia Africana is an important timber and food tree belonging to the family of Fabaceae and sub family Caesalpinaceae. The in vitro shoot regeneration potential of this species was tested using nodal and cotyledonary node explants from in vitro grown seedlings. Multiple shoots along with callus was induced from both explants - they both had potential for shoot regeneration and callus induction. However, a better response resulted from cotyledonary node explants cultured on MS media supplemented with 15mg/L BA (Benzyladenine) and 15mg/L PG (Phloroglucinol) producing a maximum of 10 shoot buds in an explant, while nodal explants responded best on MS media supplemented with 20mg/L BA producing 3 shoots. This study is the first report on the in vitro shoot regeneration from Afzelia africana.

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1 - Department Of Botany, Obafemi Awolowo University, Ile Ife, OS, 23436, Nigeria
2 - Obafemi Awolowo University, Botany, Ile Ife, Osun, 23436

Cotyledonary node
Multiple shoots
Afzelia africana.

Presentation Type: Oral Paper
Session: 44, Physiology
Location: 114/Mayo Civic Center
Date: Wednesday, July 25th, 2018
Time: 4:15 PM
Number: 44011
Abstract ID:82
Candidate for Awards:None

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