Straub, Shannon C.K. , Fishbein, Mark .
Introduction to Next Generation Sequencing.
Next generation sequencing (NGS) describes technologies that produce billions of base pairs of sequence data per experiment. The low cost of these technologies allows an increasing number of biologists to incorporate genome-scale sequencing into their research. The goal of this workshop is to introduce participants to the essentials of NGS, so that they can develop and initiate their own projects in phylogenetics, population genetics, and gene expression using this technology. In the morning, we will describe the available technologies, applications, and protocols for sample preparation and sequencing. We will also describe options for computational infrastructure and analytical pipelines. In the afternoon, participants will then have an opportunity to work with data from the Illumina platform. Topics covered during the hands-on portion will include plastome sequence assembly and probe design for nuclear gene target enrichment (Hyb-Seq). A laptop computer is required.
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1 - Hobart and William Smith Colleges, Department of Biology, 300 Pulteney Street, Geneva, NY, 14456, USA
2 - Oklahoma State University, Dept Of Botany, 301 Physical Science, Stillwater, OK, 74078, USA, 405-564-4652
next generation sequencing
Presentation Type: Workshop
Candidate for Awards:None