Abstract Detail



Systematics

Tiamiyu, Bashir Bolaji [1], Lateef, Azeez Adebola [2], Mustapha, O.T. [2], Abdulkareem, K.A. [2], Garuba, T. [2].

Morphological and Molecular characterization of Curcuma longa (Zingiberaceae) from Ilorin/FRIN, Nigeria.

DNA barcoding is a novel method of species identification based on conserved regions. Molecular techniques have evolved leading to emergence of more available DNA regions; -combining ITS, matK and psbA-trnH for phylogenetic analysis. DNA barcoding of members of Zingiberaceaein Nigeria is however far from being fully resolved. Hence the need for this work. The objectives of the study were to: (i) carry out molecular study of the plant using psbA-trnH and nuclear ITS; (ii) combine and analyse the data generated in (i) to establish phylogenetic relationship; and (iii) infer the taxonomic relationship in the plants considered. five members of the family Zingiberaceae were obtained; DNA was extracted using Zymo Research Plant Prep Kit. DNA quantification was done using UV spectrophotometer. DNA amplification was done by Polymerase Chain Reaction using ITS, matK and psbA-trnH. The sequences obtained from the sequencing company were confirmed using BLAST-n on the GenBank website (http://blast.ncbi.nlm.nih.gov) for biological sequence homology and divergence of amplified sequences. AliView version 1.17-beta1 software (Larsson, 2014) and SeqTrace version 0.9.0 (Stucky, 2012) was used to obtain a consensus sequences from the forward and reverse sequences and to edit the sequence. The DNA sequences generated in this study were submitted to GenBank to obtain their respective accession numbers. DNA sequences from this study together with reference sequences were downloaded from GenBank for phylogenetic analysis. Maximum Parsimony (MP) for phylogentic analysis was implemented in MEGA7 (Kumar, Stecher, & Tamura, 2016). Bootstrapping was done with 1000 replications. PCR amplification success rate was taken into account to assess candidate barcode regions. PCR and sequence success rate was high in psbA-trnH (60%), and ITS (10%) regions. Blast similarity percentage gave a range of 98% - 99%. The evolutionary history was inferred using the Maximum Parsimony method. Combined markers tree (Tree) gave three clades are represented with all of them showing monophyletic lineages (Clade I, and II) all with full supports of 1.0, and 0.99. There were two larger groups (Clade I and Clade III) and a smaller group (Clade II). The clade I showed a paraphyletic relation. The combined tree analysis showed that psbA-trnH is a good candidate gene for DNA barcoding of members of this family due to its PCR and sequencing success rate. The clades of the Curcuma genus showed polyphyl and these are supported by morphological characters, while the “Longa” is monophyletic, they are a morphologically distinct group.


1 - University Of Ilorin, Department Of Plant Biology, Department Of Plant Biology, Faculty Of Life Sciences, University Of Ilorin, Ilorin P.M.B 1515, Ilorin, KW, 1515, Nigeria
2 - University of Ilorin, Plant Biology

Keywords:
Curcuma
matK
psbA-trnH
ITS
Zingiberaceae.

Presentation Type: Poster This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PSY005
Abstract ID:214
Candidate for Awards:None


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