Abstract Detail



Physiology

Hilleary, Richard [1], He, Sheng Yang [2].

Temperature-Dependent Suppression of Ca2+ Signaling During Effector-Triggered Immunity in Plants.

Climate change represents a major challenge influencing current and future crop yields, not only through direct impacts of climate-associated abiotic factors on plant health, but also through concomitant stresses presented by plant pathogens. As ambient temperatures increase, the plant immune system can become compromised, making plants more susceptible to pathogen infection. Effector-triggered immunity (ETI) comprises a major node of the plant immune system and is a significant driver for the development of crop resilience to specific pathogens. ETI is dependent on the activation of nucleotide-binding leucine-rich repeat (NLR) receptors capable of recognizing the effector molecules employed by pathogens to increase their virulence. One of the earliest steps during ETI is the formation of NLR-dependent Ca2+-permeable pores in the plasma membrane, which allows Ca2+ into the cell to activate immune pathways and potentiate ETI. However, the activation of ETI in many cases is temperature sensitive and can be abolished when plants are subjected to nominal increases in ambient temperatures. We sought to characterize the impact of elevated temperature on ETI-associated immune responses via whole-plant imaging of Arabidopsis thaliana and Nicotiana benthamiana plants stably expressing genetically encoded fluorescent Ca2+ biosensors. Elevated temperature can significantly attenuate or abolish Ca2+ influx, with suppression of Ca2+ being strongly correlated with a loss of disease resistance. This research will help us understand the molecular mechanisms underlying the temperature vulnerability of NLR-mediated plant immunity and inform future breeding programs to preserve the effectiveness of NLRs in a warming climate.


1 - Duke University, Biology, 124 Science Dr, 4305 FFSC Bldg, Durham, NC, 27708, USA
2 - Duke University, 124 Science Dr, 4305 FFSC Bldg, Durham, NC, 27708, United States

Keywords:
plant-microbe interactions
climate change
plant immunity
signaling.

Presentation Type: Oral Paper
Session: PHYS1, Physiology
Location: Fireweed/Hilton
Date: Monday, July 25th, 2022
Time: 3:45 PM(EDT)
Live or Virtual Live
Number: PHYS1002
Abstract ID:245
Candidate for Awards:None


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