Abstract Detail



Comparative Genomics/Transcriptomics

Mabry, Makenzie [1], Brose, Julia M.  [2], Blischak, Paul [3], Dismukes, Wade [4], Bottoms, Christopher  [5], Edger, Patrick [6], Washburn, Jacob [7], An, Hong [5], McKain, Michael [8], Barker, Michael [9], Al-Shehbaz, Ihsan [10], Schranz, M. Eric [11], Pires, Joseph [12].

Multiple Independent Whole Genome Duplication Events in the Brassicales.

Premise of the study: Whole Genome Duplication (WGD) is prevalent throughout plants and is especially frequent in the order Brassicales, specifically, the family Brassicaceae. However, its sister family, Cleomaceae has also been characterized by a WGD, but it's placement as well as other WGD events in the order remains unclear.
Methods
: Using Phylo-Transcriptomics for 74 taxa and genome survey sequencing for 66 of those samples, we infer nuclear and chloroplast phylogenies to assess relationships among the major families of the Brassicales and within the Brassicaceae. We then use two methods of WGD inference, Ks plots and phylogenetics, to assess placement of WGD events.
Key results
: We present well-supported chloroplast and nuclear phylogenies for the Brassicales. Using the nuclear phylogeny, we successfully place several WGD events in the Cleomaceae, one in the Capparaceae, and one shared by at least two members of the Resedaceae. 
Conclusions
:  Given its economic importance and many genomic resources (i.e. well annotated small genome of Arabidopsis and many Brassica crops) the Brassicales are a perfect group to continue testing the accuracy of WGD inference methods. We add to the current conversation on WGD inference difficulties, by demonstrating that the different levels in which we sample, such as the order or family, make large differences in not only support of WGD events, but recovering them at all  


1 - University Of Missouri, Biological Sciences, 1201 Rollins St., Columbia, MO, 65201, United States
2 - Michigan State University
3 - 55 N Cherry Avenue Unit 105, Tucson, AZ, 85719, United States
4 - Iowa State University
5 - University of Missouri
6 - Michigan State University, Department of Horticulture
7 - Cornell University
8 - University Of Alabama, 411 Mary Harmon Bryant Hall, Tuscaloosa, AL, 35487, United States
9 - Department Of Ecology & Evolutionary Biology, P.O. Box 210088, Tucson, AZ, 85721, United States
10 - Missouri Botanical Garden, P.O. Box 299, St. Louis, MO, 63166, United States
11 - Wageningen University and Research, Wageningen, Netherlands
12 - University Of Missouri, 371 Bond Life Sciences Center, 1201 Rollins Street, Columbia, MO, 65211, United States

Keywords:
Brassicales
Genome Evolution
Transcriptomics
phylogeny
polyploidy.

Presentation Type: Poster This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PGT005
Abstract ID:283
Candidate for Awards:None


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