Abstract Detail



Biodiversity Informatics & Herbarium Digitization

Johnson, Bryson [1].

DNA Analysis of UVSC Herbarium Specimens and Comparison of CTAB and Kit Extractions by Bryson Johnson.

The purpose of this project is to compare DNA extraction techniques on Utah Valley University Herbarium (UVSC) specimens. Destructive samples of the plant vouchers were used to extract DNA using both CTAB and Kit extraction methods. The extracted and purified DNA samples were assessed for quantity prior to PCR amplification using a nanodrop. The ITS region of the DNA samples were then amplified through PCR and correct amplification as confirmed through gel electrophoresis. Quantity was again assessed through nanodrop. Finally, the amplified PCR products were assessed for quality by sequencing the ITS region and analyzed using the software Geneious. The goal is to find a preferable method of DNA extractions on herbarium specimens and to add the sequenced data to DNA barcoding initiatives such as the International Barcode of Life. This will add potentially valuable knowledge that can be of benefit to scientist around the world. 


1 - 1004 W Tuscany View Road D31, Midvale, UT, 84047, United States

Keywords:
Barcoding
herbarium
Sequencing
DNA Extraction.

Presentation Type: Poster This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PBI003
Abstract ID:307
Candidate for Awards:None


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