Abstract Detail



Bryology and Lichenology

Anderson Stewart , Carly Rae [1], Tripp, Erin [2].

Using Restriction Site Associated Sequencing (RADseq) Data for Phylogenetic Analysis of Cladonia lichens.

Carly R. Anderson Stewart1, Erin A. Tripp1,2  
1University of Colorado, Ecology and Evolutionary Biology Department 2University of Colorado Natural History Museum and Herbarium (COLO)
 The lichen genus Cladonia (Cladoniaceae, lichenized Ascomycota) is globally widespread, highly diverse, and ecologically important. The genus has a long history of study compared to many lichen groups due in part to its wide distribution, morphological diversity, and visual appeal. However, the drivers of diversification and biogeographical distributions remain unknown owing to the lack of a fully sampled and resolved phylogenetic history. Species estimates for Cladonia range from 400-700 taxa, making traditional methods of sequencing both cost and time-ineffective. The present study employs ddRAD–Double Digest Restriction Site Associated Sequencing–data to explore utility of these loci in resolving species-level relationships within Cladonia. Using a pilot dataset, we apply ddRAD data to Cladonia for the first time and found relationships to be both highly supported, well-resolved, and reflective of prior knowledge of the genus. This study represents one of few to have used ddRAD data to reconstruct phylogenetic history in lichens, which are highly metagenomic organisms consisting of numerous unrelated partners in high abundance within a single thallus.


1 - University of Colorado at Boulder, C105 Ramaley Hall (UCB 334), Boulder, CO, 80309, United States
2 - C105, Ramaley Hall, UCB350, Clare Small Building, Basement, Boulder, CO, 80309, United States

Keywords:
lichenized fungi
metagenomics
Phylogenetics
RADseq
bioinformatics
Cladonia.

Presentation Type: Oral Paper
Number:
Abstract ID:363
Candidate for Awards:A. J. Sharp Award


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