Abstract Detail

Functional Genetics/Genomics

Rubin, Matthew [1], Brock, Marcus [2], Davis, Seth [3], Weinig, Cynthia  [4].

QTL underlying circadian clock parameters under seasonally variable field settings in Arabidopsis thaliana.

The circadian clock facilitates coordination of the internal rhythms of an organism to daily environmental conditions, such as the light-dark cycle of one day. Circadian period length (the duration of one endogenous cycle) and phase (the timing of peak activity) exhibit quantitative variation in natural populations. Here, we measured circadian period and phase in June, July and September in three Arabidopsis thaliana recombinant inbred line populations. Circadian period and phase were estimated from bioluminescence of a genetic construct between a native circadian clock gene (COLD CIRCADIAN RHYTHM RNA BINDING 2) and the reporter gene (LUCIFERASE) after lines were entrained under field settings. Using a Bayesian mapping approach, we estimated the median number and effect size of genomic regions (Quantitative Trait Loci, QTL) underlying circadian parameters and the degree to which these regions overlap across months of the growing season. We also tested for QTL associations between the circadian clock and plant morphology. The genetic architecture of circadian phase was largely independent across months, as evidenced by the fact that QTL determining phase values in one month of the growing season were different from those determining phase in a second month. QTL for circadian parameters were shared with both cauline and rosette branching in at least one mapping population. The results provide the first insights into the QTL architecture of the clock under field settings, and suggest that the circadian clock is highly responsive to changing environments and that selection can act on clock phase in a nuanced manner.

Related Links:
Rubin et al. 2019 G3: Genes, Genomes, Genetics Early View

1 - Donald Danforth Plant Science Center, 975 North Warson Road, Miller Lab, St. Louis, MO, 63132, United States
2 - University of Wyoming, Botany, 1000 East University Ave, Laramie, WY, 82071, USA
3 - University of York, Biology, Wentworth Way, Heslington, York, YO10 5DD, UK
4 - University of Wyoming, Botany, Molecular Biology and Ecology, 1000 East University Ave, Laramie, WY, 82071, USA

Arabidopsis thaliana
circadian clock
plant shoot architecture
quantitative trait loci.

Presentation Type: Oral Paper
Number: 0004
Abstract ID:773
Candidate for Awards:Margaret Menzel Award

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