Abstract Detail



Crops and Wild Relatives

Brock, Jordan [1], Olsen, Kenneth [2].

Evolutionary origins of the emerging biofuel crop Camelina sativa.

Gold-of-pleasure (Camelina sativa) was once widely used in Europe as an oilseed crop, and has been cultivated for thousands of years for vegetable oil, lamp fuel, and animal feed. During the past 100 years, cultivation of Camelina sativa fell out of favor resulting in the loss of cultivars and genetic diversity. The newfound application of Camelina sativa oil as an aviation biofuel has spurred interest in the crop once again. However, little is known about this allohexaploid crop, or from where the subgenomes originate. We sought wild germplasm from the region of highest diversity and proposed origin in Turkey and the Caucasus. These collections represent the largest and most diverse collection of Camelina germplasm known to-date. Using a subset of 54 accessions across five known species groups, we performed genome size, phylogenetic, and genetic diversity analyses to better characterize species relatedness. A double-digest restriction-site-associated DNA sequencing (ddRADseq) approach was used to generate genome-wide markers from across 44 individuals for use in downstream analyses. Results from the phylogenetic analyses show well-supported species clades and C. sativa accessions to be nested within the C. microcarpa clade, suggesting that C. sativa is a domesticated form of the hexaploidy species C. microcarpa. Further evidence for this includes similar genome size, and a reduction in genetic diversity in C. sativa relative to C. microcarpa. We also find one accession representing a putatively new diploid species which is sister to the C. rumelica clade with strong support.


Related Links:
Personal website of Jordan R. Brock


1 - 6653 Berthold Ave. Apt 3s, St. Louis, MO, 63139, United States
2 - Washington University, Biology Dept., Campus Box 1137, 1 Brookings Drive, St. Louis, MO, 63130, United States

Keywords:
Camelina
polyploidy
Biofuel
Phylogenetics
crop wild relatives.

Presentation Type: Oral Paper
Number:
Abstract ID:166
Candidate for Awards:None


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