Abstract Detail


Dechayont, Bhanuz [1], Phuaklee, Pathompong  [1], Chunthorng-Orn, Jitpisute [1], Mokmued, Khwanchanok  [2], Poomirat, Saovapak [1], Saesiw, Ubonwan [1].

The antioxidant activity and cytotoxic activities against cancer cell lines of Thai medicinal plant recipe.

Prompak is an ancient remedy, composed of seven herbal medicines, which is wildly used in Thai folk medicine as an anticancer drug. In this study, we investigate its cytotoxic and antioxidant activities. The Prompak remedy was extracted with 95% ethanol (EtOH) and water. Cytotoxicity against three cancer cell lines as gastric cancer cell line (Kato-III), liver cancer cell line (Hep G2), and colon cancer cell line (SW 480), compared to one normal cell line as human keratinocyte cell line (Hacat) were determined by SRB assay and antioxidant activities by the ABTS and DPPH scavenging assays. The water extract had the higher yield, 4.51 %. The EtOH extract showed significant, dose-dependent killing of cancer cells including Kato III, Hep G2 and SW 480 with an IC50 of 9.04±2.22, 30.42±1.12 and 65.58±2.39 µg/mL, respectively whereas this extract was found to be non toxic to normal cells (IC50 >200 µg/mL). The water extract demonstrated higher antioxidant activities on ABTS IC50 value was 49.38±3.29 µg/mL, while the ethanolic extract displayed the highest reducing DPPH free radical with IC50 value of 12.90±1.89 µg/mL. To our knowledge, this work is the first study of the cytotoxic and antioxidant activities of Prompak remady extracts. These data support its potential use as an anticancer drug and warrant further preclinical investigation.

1 - Faculty of Medicine, Department of Applied Thai Traditional Medicine, Klong Nueang, Klong Lung, Pathum Thani, -- Country other than USA or Canada --, 12120, Thailand
2 - Division of Applied Thai Traditional Medicine, Faculty of Public Health, Naresuan University, Phitsanulok, Thailand

Prompak remedy

Presentation Type: Poster This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PET001
Abstract ID:177
Candidate for Awards:None

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