Abstract Detail



Biogeography

Willyard, Ann [1], Bower, Andrew [2], Hipkins, Valerie [3], DeWoody, Jennifer A [4].

Genetic diversity and population structure in Chrysolepis chrysophylla (giant chinquapin; Fagaceae): SSRs vs SNPs.

We used simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) genotypes on the same plant samples of Chrysolepis chrysophylla (Fagaceae; giant chinquapin) that were collected from 22 populations across its entire geographic range to investigate the genetic diversity and population structure of this tree species. We included one population of C. sempervirens that is sympatric in part of the geographic range. The SNPs were genotyped with a 2b-RAD protocol that uses Type IIB Alf1 restriction endonuclease to create uniform fragments and reduce genome complexity by selectively amplifying regions adjacent to recognition sites. Our experimental design allowed an interesting comparison of results from SSRs and SNPs for both diversity statistics and for clustering methods. There were strong northern vs. southern differences and there was also a strong pattern that grouped populations east-of the Cascade or Sierra Nevada crest separately from populations that grow west of these mountain crests. Putative gene flow between C. sempervirens and giant chinquapin was only inferred at a modest level in the Blodgett Forest site and not at other sympatric sites. Populations in two geographically isolated areas may be divergent: the Hood Canal, Washington population and three coastal southern California populations. We concluded that giant chinquapin in the far northern and far southern edges of its native range should be considered important targets for conservation.


1 - 36640 Yocum Loop, Sandy, OR, 97055, United States
2 - USDA Forest Service, Region 6, 1835 Black Lake Blvd, Olympia, WA, 98512, USA
3 - USDA Forest Service, National Forest Genetics Laboratory, 2480 Carson Road, Placerville, CA, 95667, USA
4 - USDA Forest Service, Placerville Ranger District, 4260 8 Mile Rd, Camino, CA, 95709, USA

Keywords:
RAD-seq
SNP
microsatellite
Chrysolepis
genetic diversity
Fagaceae
giant chinquapin.

Presentation Type: Oral Paper
Number:
Abstract ID:210
Candidate for Awards:None


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